Publication:

Effect of ethyl acetate extract of Melothria perpusilla on dexamethasone induced hyperglycemia in albino rats

Background Diabetes mellitus is a group of heterogeneous disorders in which carbohydrate metabolism is reduced while that of proteins and lipids is increased. Safety and tolerability factors limit the clinical use of anti diabetic drugs. The objectives of the study were to evaluate the effect of ethyl acetate extract of Melothria perpusilla (EAEMP) on dexamethasone induced hyperglycemia in albino rats.

Methods A set of six animals each weighing 110-150g were used for the experimental study. Successive tests were conducted on the same set of animals after a period of 10 days in between the drug administration. Blood was collected from the orbital sinus and fasting blood glucose levels were measured; 2% gum acacia suspension was administered in all the six animals followed by dexamethasone (0.5ml/100g) intraperitoneal injection. Blood glucose concentrations were estimated in the blood samples collected at 1h and 2h after the administration of dexamethasone administration. With the same set of animals, similar tests were repeated with the test dose of 250 mg/kg and 500 mg/kg of the ethyl acetate extract of Melothria perpusilla and glibenclamide [0.5mg/kg per oral (p.o.)].

Results Scientific data were analysed by Kruskal Wallis test. Ethyl acetate extract of Melothria perpusilla produced a significant reduction of blood glucose level when compared with control and standard.

Conclusions Treatment with Melothria perpusilla improves hyperglycaemia probably by inhibiting gluconeogenesis.