Original Research Article
A longitudinal follow-up study on sustained behavioural change and compliance post BMW management training among healthcare workers
1Department of Community Medicine, ESIC Medical College, Jaipur, Rajasthan, India
2Department of Community Medicine, Jhalawar Medical College, Jhalawar, Rajasthan, India
3National Health Mission, Uttarakhand, India
Received: 16 July 2025
Revised: 06 January 2026
Accepted: 07 January 2026
*Correspondence:
Dr. Somya Thakan,
E-mail: somyathakan@gmail.com
Copyright: © the author(s), publisher and licensee Medip Academy. This is an open-access article distributed under the terms of the Creative Commons Attribution Non-Commercial License, which permits unrestricted non-commercial use, distribution, and reproduction in any medium, provided the original work is properly cited.
ABSTRACT
Background: While training interventions on bio-medical waste (BMW) management have shown immediate improvements in knowledge and practices among healthcare workers, there is limited evidence on the sustainability of such outcomes. This study follows up with the same cohort from the 2022 intervention at three hospitals in Rajasthan to assess long-term behavioural retention and operational compliance six months post-training.
Methods: This observational follow-up study included 124 of the original 156 participants. A structured survey and observational audit were conducted to measure retention of knowledge and adherence to BMW segregation protocols, PPE usage, and reporting mechanisms for needle-stick injuries. Spot-checks and supervisor feedback were also recorded.
Results: The average knowledge score declined slightly from 16.4 (post-training) to 14.8 after six months but remained significantly higher than the pre-training baseline (9.2, p<0.001). Sustained compliance was observed in 85% for colour-coded segregation, 89% for PPE use, and 82% for hand hygiene. Reporting of needle-stick injuries, however, dropped from 89% to 63%. Class 4 and housekeeping staff showed the most retained improvement, suggesting lasting value in training lower-cadre employees.
Conclusions: While slight declines were observed, most key behaviours related to BMW management remained significantly better than baseline. This underscores the value of refresher modules and continuous monitoring to sustain impact. Structured interventions, when coupled with audits and reinforcement, can yield lasting operational changes even in low-resource hospital settings.
Keywords: Biomedical waste, Health workforce behaviour, Longitudinal study, Infection control, Sustainability
INTRODUCTION
BMW is inherently hazardous and requires systematic handling, segregation, and disposal to prevent occupational exposure, healthcare-associated infections, and environmental contamination.1 Improper biomedical waste management has been associated with needle-stick injuries, transmission of blood-borne infections, and downstream public health risks.2 Healthcare facilities generate large volumes of waste, a significant proportion of which is infectious or hazardous in nature.3 Effective biomedical waste management depends on regulatory frameworks, infrastructure, and, critically, sustained behavioural compliance among healthcare workers.4
In India, the bio-medical waste management rules, 2016 provide a comprehensive legal framework; however, implementation gaps persist due to inadequate training, inconsistent supervision, and lack of periodic reinforcement.5 Training interventions have been shown to improve short-term knowledge and self-reported compliance among healthcare workers.6,7 However, evidence on the long-term sustainability of these improvements in real-world clinical settings remains limited.8
Frontline workers, particularly housekeeping and class 4 staff, often receive minimal follow-up training despite being directly involved in waste handling.9
This study follows up on a structured BMW management training intervention conducted in 2022 across three hospitals in Rajasthan to evaluate whether observed improvements in knowledge and behaviour were sustained six months post-training.10
The study also explores areas of behavioural decay and identifies opportunities for reinforcement in low-resource hospital settings.
Objectives
To assess knowledge retention on BMW rules six months post-training. To measure sustained compliance with waste handling protocols. To evaluate gaps in implementation and potential strategies for reinforcement.
METHODS
Study design
This study adopted an observational cross-sectional follow-up design conducted on the same cohort that participated in the 2022 pre- and post-training BMW intervention. The follow-up assessed knowledge retention and sustained behavioural compliance six months after the initial training.
Study place
The study was conducted in three hospitals in Rajasthan: a General Hospital, Janana Hospital, and a Covid-designated Hospital.
Study duration
The study was conducted from July 2022 to January 2023, covering six months post-training.
Study population
The original intervention included 156 healthcare workers, out of which 124 participants were available during follow-up.
Sampling technique
Sampling was done using a complete enumeration approach, including all workers reachable at the time of follow-up. Attrition reasons included transfers, resignations, and maternity leave.
Inclusion criteria
Participants who completed both pre- and post-training assessments in 2022. Healthcare workers present in the hospital during the follow-up period. Individuals giving informed consent.
Exclusion criteria
Participants who were unavailable during follow-up due to transfer or leave. Individuals declining participation. Incomplete records in baseline datasets.
Sample size consideration
Since this was a follow-up of an established cohort, the sample size was predetermined by the number of available participants. No fresh calculation was required. Sampling followed total enumerative sampling of all accessible members of the original cohort.
Data collection procedure
Two categories of tools were used, adapted from the original study revised 20-item knowledge questionnaire assessing recall of BMW rules. Observational audit checklist evaluating correct bin colour segregation. Use of PPE during waste handling. Reporting of needle-stick injuries. Hand hygiene practices post waste handling additionally spot-checks were conducted across wards. Interviews with 12 nursing supervisors and housekeeping leads provided contextual insights and validation.
Ethical approval
Ethical clearance was obtained from the Institutional Ethics Committee prior to data collection.
Statistical analysis
Data were entered into Microsoft Excel and analyzed using SPSS version 25.
Knowledge scores were expressed as mean±standard deviation. Behavioural compliance indicators were expressed as numbers and percentages. Paired t-test was used to compare pre-training, post-training, and follow-up knowledge scores. A p value <0.05 was considered statistically significant.
RESULTS
Knowledge retention six months post-training
There was a statistically significant decline from post-training to follow-up, p=0.012, though scores remained significantly higher than baseline (p<0.001). Knowledge scores showed a statistically significant decline at six months compared to immediate post-training levels (p=0.012), but remained significantly higher than pre-training baseline values (p<0.001).
Sustained improvements were observed in segregation, PPE use, and hand hygiene, each retaining over 80 percent compliance at follow-up. Needle-stick injury reporting showed the largest decline over time. Sustained compliance was highest for PPE use and waste segregation; while reporting of needle- stick injuries showed the most pronounced decline over time.
Subgroup observations
Housekeeping staff and class 4 workers showed the highest behavioural retention. Covid Hospital workers had the best compliance, influenced by existing SOP-driven supervision. Nurses and lab technicians showed comparatively higher regression in compliance behaviours.
DISCUSSION
This longitudinal assessment indicates that structured BMW training leads to meaningful and sustainable behavioural improvements among healthcare workers, even six months after the intervention. Despite minor regression, most compliance indicators remained substantially higher than baseline. This aligns with previous research showing that while knowledge may decline without reinforcement, trained behaviour tends to persist where institutional norms are strong.1
The findings demonstrate that structured BMW management training can result in sustained behavioural change six months post-intervention, particularly when reinforced through institutional norms and supervisory oversight.1 Although a modest decline in knowledge scores was observed, compliance with critical practices such as segregation and PPE use remained above 80 percent.2
The decline in needle-stick injury reporting highlights a gap in sustaining procedural behaviours that lack immediate perceived benefit, underscoring the need for targeted reinforcement strategies.3 Similar longitudinal studies have reported partial regression in the absence of refresher training, supporting the importance of continuous audit and feedback mechanisms.4-6
Consistent with prior research, this study reinforces that policy awareness, supervision, and systemic support are key determinants of long-term compliance.7-9 The stronger retention among housekeeping and Class 4 staff suggests that focused, task-specific training may yield durable behavioural outcomes.10
Limitations
The study showed attrition in follow-up limits representativeness. Observational audit may still have an observer effect (Hawthorne bias). No biochemical markers or actual infection incidence were measured.
CONCLUSION
Structured BMW training programs yield long-term benefits, but some behaviour decay is natural. BMW training demonstrates clear long-term benefits, with most behaviours sustaining over six months. Integrating refresher training, supervisory monitoring, and systemic reinforcement mechanisms such as audit loops can help institutionalize safe waste management practices across healthcare settings. This follow-up study advances the field by highlighting the importance of longitudinal tracking in public health intervention evaluation.
Funding: No funding sources
Conflict of interest: None declared
Ethical approval: The study was approved by the Institutional Ethics Committee
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