Original Research ArticleInternational Journal of Research in Medical Sciences
Publication: Sperm penetration assay and its correlation with semen analysis parameters
Int J Res Med Sci. 2015;3(11):3197-3201.DOI10.18203/2320-6012.ijrms20151162
Abstract
Background Aim of current study was to determine whether the Sperm Penetration Assay (SPA) can be used as a test to discriminate the infertile male from fertile one. We have also correlated the SPA with semen analysis.
Methods Sperm characteristics namely Semen analysis and the sperm penetration assay were tested in 44 infertile and 10 fertile men. Sperm penetration assay was determined by using zona free hamster eggs.
Results With decreasing spermatozoa concentration in the semen there was significant decrease in percentage penetration of zona free Hamster eggs (p 0.05).
Conclusions The Sperm penetration assay could discriminate the infertile group from fertile group significantly (p<0.001). The test appeared to be highly reproducible and probably identifies a truly infertile male.
Keywords
Affiliations
- Department of Obstetrics & Gynaecology, Institute of Medical Sciences, BHU, Varanasi, U.P.
- Department of Obstetrics & Gynaecology, SGP, Kent
- Department of Microbiology, Institute of Medical Sciences, BHU, Varanasi, U.P.