Publication:

From pathogen to protection: assessment of vaccination of fish

Background Common pathogens in the fisheries sector are primarily bacterial contaminants that are the sources of severe economic losses due to high mortality rates, with Aeromonas, Staphylococcus, Vibrio, Pseudomonas species are primarily very pathogenic to farm fishes in India.

Methods Experimental fish were vaccinated with bath immersion method and injected intra-peritoneal route with subsequent experimental infection. The vaccine was evaluated and the prepared vaccine was tested for sterility.

Results Treatment i.e. polyvalent T5, T6 and survival (after challenge) of experimental group obtained using Fisher’s exact test. The resulting p value of 0.0084 (p<0.05) indicated statistically substantial association between treatment type and median number of survivals. Vaccine type T6 has significantly greater number of survivals as matched to controls.

Conclusions Experimental trials of formalin inactivated vaccine via bath immersion were found to be much more effective and significant in fingerlings stage while in case of adult fish, result of intraperitoneal injection was observed as significant. As results revealed that vitamin C along with T5 i.e. T6, might have been established more immunity than others formulation doses.