Publication:

Restoring natural hair pigmentation: clinical assessment of safety and efficacy of an ayurvedic leave-on treatment

Background This clinical study evaluated the safety and efficacy of an Ayurvedic leave-on treatment for premature hair greying in healthy Indian adults (male and female). It also investigated the product’s mechanism of action through in vitro analyses.

Methods Healthy male and female volunteers aged 20–42 years with early-onset hair greying and no underlying medical conditions were enrolled in the clinical study. Participants were screened based on predefined criteria, including a specific percentage of grey hair. The study included a washout period before treatment initiation, followed by a four-month application phase with regular follow-ups. An in vitro study using B16F10 melanocytes (ATCC) was also conducted to explore the Ayurvedic leave-on treatment’s mechanism of action.

Results The Ayurvedic leave-on treatment significantly increased natural black hair density and reduced grey hair count in both male and female subjects. In vitro analysis reveals that the Ayurvedic leave-on treatment enhances melanogenesis by upregulating melanin production in melanocytes.

Conclusions The present study concludes that the Ayurvedic leave-on treatment promotes melanogenesis, reduces oxidative stress, provides cytoprotective benefits, and effectively enhances dark hair density while reducing grey hair count. Clinically, 100% of users showed improved dark hair density, 97% reported reduced grey hair. No adverse effects were observed, confirming its safety and efficacy for both men and women with greying concerns.