Publication:

Prevalence of carbapenem-resistant isolates from clinical samples in a tertiary care hospital Vadodara, Gujarat

Background Carbapenem resistance in Gram-negative bacilli is a major concern due to the increasing antibiotic resistance, treating infections caused by Enterobacteriaceae has become more challenging. Objective was to investigate the prevalence of blaNDM, blaOXA, blaKPC, blaIMP, blaVIM producing carbapenem-resistant Enterobacteriaceae and non lactose fermenter.

Methods The prospective observational study was   conducted   from September 2025 to October 2025 at department of microbiology, Parul Institute of Medical Sciences and Research. Total 100 samples were identified by VITEK 2. A modified carbapenem inactivation method was performed to detect presence of carbapenemase. O.K.N.V.I resist-5 is a rapid in vitro multiplex immunoassay kit used for the phenotypic detection of blaKPC, blaOXA, blaVIM, blaIMP, blaNDM directly from bacterial colonies.

Results A total 100 clinical samples were taken from that 60 (60%) Enterobacteriaceae and non lactose fermenter analysed by VITEK 2. Out of them 25 (41%) isolates were multidrug resistant carbapenem Enterobacteriaceae. From 25 (41%) samples 10 (40%) were urine samples, followed by 5 (20%) blood culture, 5 (20%) pus discharge, 2 (8%) drain fluid, 2 (8%) were ET, 1 (4%) CSF. Out of 25 MDR Enterobacteriaceae and non lactose fermenter 10 (40%) were Klebsiella pneumoniae isolated 4 blaNDM, 1 OXA, 5 blaNDM and blaOXA, followed by 6 (24%) E. coli isolated 1 blaNDM, 1 blaOXA, 4 blaNDM and blaOXA, 3 (12%) Pseudomonas aeruginosa isolated 1 blaVIM and 2 blaNDM, 4 (16%) Acinetobacter baumannii isolates 4 blaNDM, 1 (4%) Acinetobacter lwoffii blaVIM gene was detected.

Conclusions Knowledge of the prevalence of genes responsible for carbapenemase in a particular region will help in antibiotic stewardship for the selection of empiric antibiotics.