Publication:

Effect of ticlopidine on acute and subacute models of inflammation in male wistar rats: an experimental study

Background Atherosclerosis and its complications remains the major cause of death and premature disability. Atherogenesis involves elements of inflammation, a process that now provides a unifying theme in the pathogenesis of the disease. Anti-platelet drugs are currently used in the treatment of atherosclerosis and its complications. Our study evaluated the influence of ticlopidine on acute and sub-acute models of inflammation in male Wistar rats.

Methods Male wistar rats (150-200g) were divided into three groups i.e. control, Aspirin and ticlopidine (n=6 animals in each group). The effect of ticlopidine, administered orally, on inflammation was studied using acute (carrageenan induced rat paw edema) and sub-acute (cotton pellet granuloma and histopathological examination of grass piths) models. Experiment was conducted according to the Committee for the Purpose of Control and Supervision on Experiments on Animals (CPCSEA) guidelines. Analysis was done using one way ANOVA followed by Post Hoc Test of Dunnets. P<0.05 was considered as statistically significant.

Results Ticlopidine showed significant inhibition of rat paw oedema in acute model (P<0.01) and granuloma dry weight, in subacute model of inflammation when compared to control (P<0.01). Histopathological examination of grass pith revealed markedly reduced fibroblasts, granulation tissue, fibrous tissue and collagen in ticlopidine group when compared to control.

Conclusions Ticlopidine exhibited a significant anti-inflammatory activity in acute and sub-acute models of inflammation.