Publication:

High performance thin layer chromatography analysis of seed extracts of Tudri Surkh (Cheiranthus cheiri)

Background To develop the high-performance thin layer chromatography (HPTLC) finger print profile of hydroalcoholic and ethylalcohol extracts of seeds of Tudri Surkh (Cheiranthus cheiri).

Methods Chromatographic technique was used for separation of components from hydroalcoholic and ethylalcohol extracts of seeds and HPTLC was carried out using CAMAG HPTLC system equipped with Linomat V applicator, and WinCATS software.

Results HPTLC profiling of the extract confirm the presence of various phytochemicals. At 640 nm HPTLC finger print of hydroalcoholic extract revealed 16 components with Rf=0.05-0.94 while ethylalcohol extracts revealed 19 components peaks with Rf=0.07 to 0.96. Well separated and compact greenish, yellow and pink bands were visualized using anisaldehyde sulphuric acid reagent.

Conclusions We conclude that HPTLC fingerprint profiling of seed extracts of Tudri Surkh (Cheiranthus cheiri) revels presence of various compounds and can be utilized as a marker for standardization and proper identification of the plant material to be used for preparation of traditional drugs.