Publication:

Study of morphological spectrum of gallstone and bacteriology of bile in cholelithiasis

Background Biliary calculus disease is one of the most common disorders of gastrointestinal tract. Gall bladder stone are crystalline structure formed by concretion of bile constituents. Aetiology of gall stone is multifactorial. Bile is found to be sterile in normal patient.

Methods This is a cross sectional study conducted in department of surgery, Lt Lakhiram Agrawal Memorial Medical College, Raigarh, Chhattisgarh, India from October 2015 - October 2016. Total 50 cases were selected and operated by lap /open cholecystectomy were included in this study. During cholecystectomy bile was aspirated and was sent to laboratory for culture. Gallstone retrieved from the specimen was classified based on morphological appearance.

Results Gallstone disease found to be common in female with ratio of 4:1, with maximum no. of cases found in the age group of 35 to 45 year. Bile culture as positive in 40%. The most common organism was E. coli isolated in 14 cases, followed by Klebseilla in 4 cases.

Conclusions The frequency of bile infection in this study area was 40%, in which association with mixed type gallstone was found to be significant and the most common bacteria isolated was E. coli.