Publication:

Screening of novel 2-4methylphenylimino-3-carboxamide substituted thiophene compound for peripheral analgesic activity

Background Pain is an unpleasant sensation that can range from mild, localized discomfort to agony. The mechanism of pain perception involves dedicated subsets of peripheral and central neurons. For management of pain, currently, available treatment regimens are opioid analgesics and NSAIDs which are easily available over the counter drugs. Long term use of analgesics can lead to significant impact on human renal system, cardiovascular system and analgesic abuse etc. So, there is a need for novel, safe and cost effective analgesic compound. Hence a study on 2-4methylphenylimino-3-carboxamide substituted thiophene compound, a novel thiophene compound has been carried out in different experimental animal models.

Methods Two methods were used to evaluate the peripheral analgesic activity of 2-4methylphenylimino-3-carboxamide substituted thiophene compound. First method was Acetic acid induced writhing and compared to standard drug aspirin. Second method was late phase of formalin induced paw licking in mice and compared to a standard drug, aspirin.

Results With Acetic acid induced writhing, 40mg/kg dose of 2-4methylphenylimino-3-carboxamide substituted thiophene compound has shown maximum Pain Inhibition Percentage (PIP) of 78 % compared to 91% by Aspirin. Whereas with late phase of Formalin test, 40mg/kg dose of 2-4methylphenylimino-3-carboxamide substituted thiophene compound has shown maximum PIP of 58% in late phase compared to 86% by aspirin. The results were statistically significant with p<0.05.

Conclusions It can be concluded that 2-4methylphenylimino-3-carboxamide substituted thiophene compound found to have moderate peripheral analgesic activity as evident in acetic acid induced writhing and late phase of formalin tests.