Publication:

Evaluation of antidiabetic activity of Ocimum sanctum (Tulsi) through inhibition of alpha-amylase and alpha-glucosidase: an in vitro study

Background The antidiabetic potential of Ocimum sanctum (Tulsi) has been of increasing interest due to its traditional use in herbal medicine. The aim of this study was to evaluate the inhibitory effects of Ocimum sanctum extracts on alpha amylase and alpha glucosidase enzymes and compare these effects to those of the standard drug, acarbose.

Methods Various concentrations of Ocimum sanctum extracts were prepared and tested for their inhibitory activity against alpha-amylase and alpha-glucosidase enzymes. The inhibitory effects were measured and compared to the effects of acarbose, a known antidiabetic drug. Results The extracts of Ocimum sanctum demonstrated a significant inhibitory effect on the alpha-amylase enzyme, with inhibition percentages of 9%, 16.6%, 28.5%, 41.1%, and 47.3% at concentrations of 20, 40, 60, 80, and 100 µg/ml, respectively. However, no significant inhibitory effect was observed on the alpha-glucosidase enzyme when compared to acarbose.

Conclusions Ocimum sanctum shows potential as an antidiabetic agent through its significant inhibitory effect on alpha-amylase. Further research is needed to explore its full potential and mechanism of action.