Publication:

Unraveling Iris ensata Thunb: a pharmacognostical, physicochemical and HPLC-assisted phytochemical investigation

Background Iris ensata Thunb, commonly known as the Japanese iris, is a species of flowering plant in the Iris family (Iridaceae). Native to Japan, China, Korea, and Russia, it thrives in wetland environments and is often found near ponds and streams.

Methods To standardize the test drug, various analytical techniques were employed, including organoleptic evaluation, extractive value determination, ash content analysis, phytochemical screening, elemental analysis, fluorescence study, and HPLC profiling.

Results The findings revealed that the total ash content was 6.98%, while the water-soluble and alcohol-soluble extractive values were 13.3% and 9.81%, respectively. The loss on drying was recorded at 4.21%, foreign matter was 0.66% and the bulk density was found to be 0.63%. The HPLC analysis displayed 18 peaks, with peak 02 and peak 03 being the most significant, showing area concentrations and retention times of 54.676% at 2.828 minutes and 43.822% at 3.051 minutes, respectively.

Conclusions This study provided essential reference data for ensuring the quality, purity, and identification of future batches of I. ensata Thunb contributing to its standardization in medicinal applications.