Publication:

Study of the renoprotective effect of Citrus limon juice and Emblica officinalis extract on renal toxicity induced by carbon tetrachloride in Wistar rats

Background The renoprotective effects of Citrus limon juice and Emblica officinalis are not considered till now. Both of them have a strong antioxidant effect.

Methods Seven groups with six rats in each group. I: Normal control (1 ml distilled water/oral route), Group II: disease control (1 ml distilled water/oral route), group III: Acetylcystine (950 mg/kg), Group IV: Citrus limon (6 ml/kg/oral route), group V: Emblica officinalis (700 mg/kg/oral), Group VI: Citrus limon with Emblica officinalis (6 ml/kg + 700 mg/kg/oral route) Group VII-Citrus limon with Emblica officinalis and acetylcystine (6 ml/kg + 700 mg/kg + 950 mg/kg oral). Only a single dose of CCl₄ was given by intraperitoneal injection of 1.5 ml/kg of 20% carbon tetrachloride dissolved in olive oil for induction of renal injury. One-way ANOVA and paired t-tests were used to analyze data.

Results The BUN of animals in standard control (Group III) and Citrus limon juice with Emblica officinalis alone and in combination (Groups IV, V, and VI) and in combination with the standard drug and Citrus limon juice with Emblica officinalis test groups was significantly lower than the disease control group (Group II) (p<0.05). Effect on serum creatinine: As seen, serum creatinine was significantly reduced in the positive control (Group III) as compared to the disease control (Group II 4.3) (p<0.001).

Conclusions The present study demonstrated the renoprotective effect of citrus limon juice and Emblica officinalis in Wistar rats.